Effect of Kidney-tonification, Spleen-invigoration and Detoxification Methods on T lymphocytes Mediated by Peripheral Blood DCs in Chronic HBV Infection Patients
10.11842/wst.2013.05.005
- VernacularTitle:补肾、健脾、解毒法对慢性乙型肝炎患者外周血DCs介导的T淋巴细胞的影响
- Publication Type:Journal Article
- Keywords:
Chronic HBV infection;
kidney-tonification;
spleen-invigoration;
detoxification;
dendritic cells (DCs);
T lymphocytes
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2013;(5):825-830
- CountryChina
- Language:Chinese
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Abstract:
This study was aimed to compare the effect of traditional Chinese medicine ( TCM ) treatment method of kidney-tonification, spleen-invigoration and detoxification on T lymphocytes of patients with chronic HBV infec-tion, which were mediated by peripheral blood dendritic cells (DCs). Ten patients with chronic HBV infection and
eight healthy controls were included in this study. DCs were differentiated from PBMCs which were isolated from their peripheral blood, incubated and induced. Then, the DCs were interfered by plasma of medicine through the treatment methods of kidney-tonification, spleen-invigoration and detoxification. Models of interaction were estab-lished. Expressions of CD3+, CD4+, CD8+, CD8+CD28+, CD8+PD-1 of DCs and T cells were detected by FACS; and the levels of IFN-γ in supernatants were detected by ELISA. The results showed that the kidney-tonification plasma, spleen-invigoration plasma, detoxification plasma deal with HBcAg in load DCs incubated T lymphocytes. The CD3+ and CD4+ expression rate were increased; the CD8+ and CD8+PD-1 expression were reduced; IFN-γ level was increased; but only the Liuweidihuang Tang group was with significant difference compared to the model group (P < 0.05); the CD8+CD28+ expression rate was increased in the Sijunzi Tang and Liuweidihuang Tang group compared to the model group ( P < 0 . 05 ) . It was concluded that to a certain extent , the treatment method of kidney-tonification, spleen-invigoration and detoxification can restore the function of peripheral blood DCs in chronic HBV infection patients in order to restore its downstream T lymphocyte function. Among these treatment methods, the optimal method is kidney-tonification.
