Deguelin treatment delays embryonic development of zebrafish:A gene chip analysis
10.3969/j.issn.2095-4344.2013.37.011
- VernacularTitle:基因芯片分析鱼藤素对斑马鱼胚胎发育的影响
- Author:
Wenhao LI
;
Ruijin LIU
;
Xinrong WU
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2013;(37):6607-6612
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Anticancer drug deguelin delays the embryonic development of zebrafish, but its exact mechanism is not yet clear.
OBJECTIVE:To explore expression differences in genes and signaling pathways in deguelin-treated zebrafish embryos.
METHODS:Zebrafish embryos were harvested from zebrafish fed according to the Zebrafish Book. Zebrafish embryos at 2-cel stage were selected and divided into two groups:control group treated with dimethyl sulfoxide incubator liquid, and experimental group treated with 0.6μmol/L deguelin. Total RNA was extracted from the two groups at sphere stage. Then, gene chip technique was used to detect differential y expressed genes in the deguelin-treated zebrafish embryos. Real-time quantitative PCR was employed to validate microarray cluster analysis and pathway analysis to explore the mechanism of action of deguelin.
RESULTS AND CONCLUSION:Chip results showed that 407 genes were upregulated more than three times and 461 genes were downregulated more than three times after deguelin treatment. PCR validation results were consistent with those of the chip. Fourteen pathways were identified by KEGG pathway analysis. Deguelin may play an important role by intervening cel metabolism growth and differentiation.
