Eye acupuncture therapy affects intercellular adhesion molecule 1 expression in rat hippocampus of acute cerebral ischemia-reperfusion injury
10.3969/j.issn.2095-4344.2013.37.015
- VernacularTitle:眼针治疗急性脑缺血再灌注损伤大鼠海马组织细胞间黏附因子1的变化
- Author:
Qian PAN
;
Zhe WANG
;
Yuan GAO
;
Dan YU
;
Ying WANG
;
Huan JING
;
Shouyan WANG
;
Jian WANG
;
Hongquan GUAN
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2013;(37):6636-6640
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Significant increasing of intercel ular adhesion molecule 1 expression can promote the occurrence of inflammatory response and increase brain tissue injury.
OBJECTIVE:To investigate the effect of eye acupuncture point and non-point therapy on intercel ular adhesion molecule 1 expression in rat hippocampus of acute cerebral ischemia-reperfusion injury model.
METHODS:Forty Sprague Dawley rats were divided into normal group, sham-operation group, model group, eye acupuncture point group and eye acupuncture non-point group. Rats in the model group, eye acupuncture point group and eye acupuncture non-point group were used to establish the acute cerebral ischemia-reperfusion injury model with suture method, the suture was deep for 1.8-2.2 cm. In the eye acupuncture point group, the acupuncture points of hepatic region, upper energizer area, lower energizer area and kidney area were selected when cerebral ischemia-reperfusion occurred immediately and 30 minutes before drawing materials, then horizontal needling the acupuncture points around the orbit for 20 minutes. In the eye acupuncture non-point group, the puncture site was selected at 3 mm away from eye acupuncture non-point area, and then needled the puncture site with the same method in the eye acupuncture point group.
RESULTS AND CONCLUSION:After eye acupuncture point therapy, the neurological deficit scores were decreased, and the intercel ular adhesion molecule 1 expression in rat hippocampus was significantly decreased (P<0.01). After eye acupuncture non-point therapy, there were no significant changes in neurological deficit scores and the expression of intercel ular adhesion molecule 1 protein and mRNA in rat hippocampus. The results indicate that eye acupuncture can significantly improve the rat cerebral ischemia-reperfusion injury, and the mechanism may relate with reducing the intercel ular adhesion molecule 1 expression in rat hippocampus.
