Platelet-rich plasma affects the proliferation and collagen production of mesenchymal stem cells
10.3969/j.issn.2095-4344.2013.36.019
- VernacularTitle:富血小板血浆与间充质干细胞增殖和胶原的产生
- Author:
Chun RONG
;
Yanqing SHEN
;
Yaqing Lü
;
Mingyu LI
;
Changsuo XIA
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2013;(36):6501-6507
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Mesenchymal stem cel s are the seed cel s for tendon tissue engineering which can be obtained in large quantities, but how to induce in vitro is a key technology.
OBJECTIVE:To explore the effect of platelet-rich plasma on the proliferation and col agen production of in vitro cultured mesenchymal stem cel s.
METHODS:The rabbit mesenchymal stem cel s were separated ad cultured. The high-dose platelet-rich plasma group, middle-dose platelet-rich plasma group and low-dose platelet-rich plasma group were set to induce the mesenchymal stem cel s, and the blank control group was set as control.
RESULTS AND CONCLUSION:The proliferation of mesenchymal stem cel s in the high-dose platelet-rich plasma group, middle-dose platelet-rich plasma group and low-dose platelet-rich plasma group was high, and in rapid growth with big increase amplitude, and there was no significant difference in the proliferation when compared with the blank control group (P<0.05). The effect was positively correlated with the culture time, and after cultured for a certain time, the effect was in dose-dependent manner, as higher dose platelet-rich plasma had more significant effect on the proliferation of the cel s. The results indicate that platelet-rich plasma can significantly promote the synthesis of col agen type Ⅰ and Ⅲ of mesenchymal stem cel s, the higher the dose, the more significant the effect on the col agen.
