Study on the genetic characteristics and molecular epidemiology of Echovirus 11 strains isolated from patients with hand food and mouth disease in He-nan province, China
10.3760/cma.j.issn.0254-5101.2013.09.010
- VernacularTitle:手足口病例中埃克病毒11型河南株分子流行病学研究
- Author:
Yuling XU
;
Haiyan WEI
;
Haomin CHEN
;
Xueyong HUANG
- Publication Type:Journal Article
- Keywords:
HFMD;
Echovirus 11;
Genetic characteristics
- From:Chinese Journal of Microbiology and Immunology
2013;(9):688-691
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the evolution and genetic characteristics of Echovirus 11 (Echo11) strains isolated from patients with hand food and mouth disease ( HFMD) in Henan province. Methods Enterovirus strains were isolated from stool samples of patients with HFMD from year 2010 to 2012.The sequences of VP1 region of all positive strains were amplified , sequenced and then analyzed by BLSAT to determine their genotypes .The entire VP1 coding regions of 10 Echo11 strains were amplified by RT-PCR, and the homology analysis was conducted among them and other Echo 11 strains published in Gen-Bank.A phylogenetic tree was constructed to evaluate the evolution of Henan isolates and the prevalence of different genotypes .Results From year 2010 to 2012 , 184 non-Enterovirus 71 ( EV71 ) strains and non-Coxsakievirus A16 (CA16) strains causing HFMD were isolated, 10 (5.43%) of with were Echo11 strains. There were 876 nucleotides ( nt) in the complete VP1 gene sequence , encoding 292 amino acids ( aa) .The 10 Echo11 strains shared 93.1%-100% homologies in nt sequences and 97.3%-100% in aa sequences. The homology analysis indicated that Henan strains and prototype strains were highly similar but different with the homology of 77.8%-78.8% in nt and 90.8%-91.8% in aa, respectively.Conclusion Echo11 was one of the pathogens causing HFMD in He-nan province and genotype A was the dominant genotype .
