Relationship between ZNF139 and multidrug resistance(MDR) related genes in SGC7901 and SGC7901/ADR cell lines
10.3969/j.issn.1007-3969.2013.07.003
- VernacularTitle:人胃癌耐药细胞株中锌指蛋白139和多药耐药基因表达的关系及意义
- Author:
Yong LI
;
Qun ZHAO
;
Bibo TAN
;
Liqiao FAN
;
Qingwei LIU
;
Zhikai JIAO
;
Xuefeng ZHAO
;
Yingjie HAO
- Publication Type:Journal Article
- Keywords:
Zinc ifnger protein 139;
Gastric cancer;
Multidrug resistance
- From:China Oncology
2013;(7):493-498
- CountryChina
- Language:Chinese
-
Abstract:
Background and purpose:It was reported that zinc ifnger protein 139 (ZNF139) was expressed aberrantly in gastric cancer. But the relationship between ZNF139 and multidrug resistance (MDR) of gastric cancer is still not clear. The purpose of this research was to investigate the expressions and signiifcance of ZNF139, MRP-1, MDR1/P-gp, GST-π in human gastric carcinoma cell lines SGC7901 and SGC7901/ADR. Methods: The expressions of ZNF139, MRP-1, MDR1/P-gp, GST-πwere determined with RT-PCR and Western blot in SGC7901 and SGC7901/ADR cell lines. Then siRNA recombinant plasmid of targeting ZNF139 gene was constructed and imported into gastric cancer cell line SGC7901/ADR, and the expressions of MRP-1, MDR1/P-gp, GST-πwere tested simultaneously. Results:The expressions of ZNF139, MRP-1, MDR1/P-gp, GST-πwere higher in SGC7901/ADR than in SGC7901(P<0.05). ZNF139 was inhibited obviously after siRNA-ZNF139 was transfected into SGC7901/ADR, and expression of MRP-1, MDR1/P-gp, GST-πdecreased(P<0.05). Conclusion:ZNF139 may be invovled in multidrug resistance (MDR) of gastric cancer by up-regulating MRP-1, MDR1/P-gp and GST-π.
