Content Determination of TancantongⅡA and Cryptotanshinone in Zhiwei Fuwei Wan by RP-HPLC
10.3969/j.issn.1005-5304.2013.08.019
- VernacularTitle:RP-HPLC测定制萎扶胃丸中隐丹参酮和丹参酮ⅡA含量
- Author:
Xiaoshuan LIU
;
Zhenghua JIAO
;
Xixiang LI
;
Siyu XU
- Publication Type:Journal Article
- Keywords:
Zhiwei Fuwei Wan;
cryptotanshinone;
Tanshinone ⅡA;
RP-HPLC
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2013;(8):54-55
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the method for determining the content of cryptotanshinone and tanshinone ⅡA in Zhiwei Fuwei Wan. Methods The determination of cryptotanshinone and tanshinone ⅡA was performed on Symmetry C18 column with mobile phase consisted of acetonitrile-0.2%phosphate (60∶40) at flow rate of 1.0 mL/min. The detection wavelength was set at 262 nm. Results The linear ranges of cryptotanshinone and tanshinone ⅡA were 0.081 4-0.407 2 μg (r=0.999 7) and 0.163 6-0.81 8 μg (r=0.999 5) respectively, the average recoveries were 99.70% (RSD=1.37%) and 99.22% (RSD=0.94%) respectively. Conclusion The method is simple and rapid for the quality control of the product.
