Qualitative Analysis of Composition in Water Extracts from Hugu Capsule by HPLC
10.3969/j.issn.1005-5304.2013.08.025
- VernacularTitle:护骨胶囊水提液HPLC图谱特征分析
- Author:
Baohong LI
;
Jindong WU
;
Wenchang ZHAO
;
Lijun SONG
- Publication Type:Journal Article
- Keywords:
water extracts from Hugu capsule;
HPLC;
qualitative analysis;
analysis on chromatographic fingerprint
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2013;(8):69-71
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze and identify the chemical composition in water extracts from Hugu capsule and provide evidence for its pharmacological study and quality control. Methods A HPLC-DAD method was used. The separation was performed on Kromasil C18 column with acetonitrile and 1%glacial acetic acid as mobile phase by gradient elution at a flow rate of 0.8 mL/min. The chemical composition in water extracts from Hugu capsule were identified by comparison with the related chromatographic fingerprint. Results Thirteen characteristic peaks were found in the HPLC chromatographic fingerprint, and four peaks were identified. Conclusion The HPLC-DAD fingerprint expressed the general character of the chemical composition in water extracts from Hugu capsule. It can be used for qualitative analysis of water extracts from Hugu capsule, and improve the quality of Hugu capsule.
