Effects of H2O2 and chlorhexidine on MMP-1, TIMP-1,2, Type 1 collagen, fibronectin and UNCL expressions in human periodontal ligament fibroblasts.
10.5051/jkape.2008.38.4.645
- Author:
Seong Mi CHOI
1
;
Hyun Seon JANG
;
Byung Ock KIM
Author Information
1. Department of Periodontology, School of Dentistry, Cho Sun University, Korea. bobkim@chosun.ac.kr
- Publication Type:Original Article
- Keywords:
chlorhexidine;
H2O2;
human periodontal ligament fibroblasts
- MeSH:
Chlorhexidine;
Collagen Type I;
Fibroblasts;
Fibronectins;
Humans;
Matrix Metalloproteinase 1;
Periodontal Ligament;
RNA, Messenger;
Tissue Inhibitor of Metalloproteinase-1
- From:The Journal of the Korean Academy of Periodontology
2008;38(4):645-656
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To evaulate the effects of chlorhexidine and H2O2 on matrix metalloproteinase-1 (MMP-1), tissue inhibitor of metalloproteinase(TIMP-1, TIMP-2), Type 1 collagen, fibronectin and UNCL expressions in human periodontal ligament fibroblasts (hPDLF). MATERIALS AND METHODS: 1.2x10(-1)%, 1.2x10(-2)% and 1.2x10(-3)% CHX and 3x10(-3)%, 3x10(-4)% and 3x10(-5)% H2O2 and mixture of CHX and H2O2 were applied to hPDLF for 1 min and 30 min. The mRNA expressions of MMP-1, TIMP-1 and 2, Type 1 collagen, fibronectin and UNCL in hPDLF were analysed by RT-PCR. RESULTS: The result were as follows: 1. The expression of UNCL mRNA was higher than that of other mRNAs. 2. 1.2x10(-3) % CHX increased mRNA expressions of hPDLF as application time increased. 3. H2O2 lower than 3x10(-3) % increased expression of UNCL mRNA, and did not decrease mRNA expression of hPDLF. 4. hPDLF treatment with 1.2x10(-1) % CHX (with or without H2O2) resulted in no gene expression. 5. hPDLF treatment with 1.2x10(-2) % CHX (with or without H2O2) for 30 minutes resulted in no gene expression. CONCLUSION: Because low concentration of CHX and H2O2 increased UNCL mRNA expression of hPDLF, low concentraction of CHX and H2O2 may have an antioxidative effect.
