Comparison of Inhibitory Activity between Daidzein and Puerarin on LPS Induced NO Production in RAW264 . 7 Cells

VernacularTitle:大豆素与葛根素抑制LPS诱导RAW264.7细胞NO产生的活性比较
Author: Ming ZHANG ; Di WANG ; Yizeng WAN ; Hao AI ; Junxiang LI ; Jian LI
Publication Type:Journal Article
Keywords: Daidzein; Puerarin; RAW264 . 7; NO
From: World Science and Technology-Modernization of Traditional Chinese Medicine 2013;(4):648-652
CountryChina
Language:Chinese
Abstract: This study was aimed to compare the activation of daidzein and puerarin on antioxidation, NO and NOS suppression in vitro . The RAW264 . 7 cell line was used to prepare radical reaction model induced by LPS (1 μg/mL). MTT method was adopted to detect cytotoxicity of daidzein and puerarin. The DCFH-DA probe and confocal microscopy were used to examine the antioxidant ability of daidzein and puerarin. Griess reagent was adopted to test the NO level in the culture medium. And chemical colorimetry was used to detect the content in RAW264.6 cells. The results showed that daidzein and puerarin can significantly suppress the NO and T-NOS expression in RAW264.7 cells induced by LPS. It was concluded that there was no difference on the activation of antioxidant free radical between daidzein and puerarin . In this regard , daidzein can be the used as substitutes of puerarin .