Visualization of periodontopathic bacteria within crevicular epithelial cells with fluorescence in situ hybridization.
10.5051/jkape.2008.38.4.691
- Author:
Youngkyung KO
1
Author Information
1. Department of Dentistry, School of Medicine, The Catholic University, Korea. ko_y@catholic.ac.kr
- Publication Type:Original Article
- Keywords:
bacterial invasion;
Porphyromonas gingivalis;
Tannerella forsythia;
gingival epithelial cells
- MeSH:
Bacteria;
Chronic Periodontitis;
Collodion;
Connective Tissue;
Epithelial Cells;
Epithelium;
Fluorescence;
Forsythia;
Humans;
In Situ Hybridization;
Periodontitis;
Porphyromonas gingivalis
- From:The Journal of the Korean Academy of Periodontology
2008;38(4):691-698
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Periodontal pathogens can invade the host tissue. Morphologic studies have revealed bacteria within the pocket epithelium, gingival connective tissues, alveolar bone, and oral epithelium. The objective of this study was to visualize and evaluate presence of Porphyromonas gingivalis and Tannerella forsythia in crevicular epithelial cells of periodontally healthy subjects and chronic periodontitis patients. MATERIALS AND METHODS: A total of 666 crevicular epithelial cells in the samples obtained from 27 chronic periodontitis patients and 9 healthy volunteers were examined. Specific probes for P. gingivalis and T. forsythia and a universal probe for detection of all eubacteria targeting 16S rRNA for fluorescence in situ hybridization was used in conjunction with confocal laser scanning microscopy. RESULTS: 98.99% of sulcular epithelial cells from healthy volunteers and 84.40% of pocket epithelial cells from periodontitis patients were found to harbor bacteria. P. gingivalis and T. forsythia were discovered more often in crevicular epithelial cells from periodontitis patients. CONCLUSION: P. gingivalis and T. forsythia can invade crevicular epithelial cells and intracellular bacteria may act as a source of bacteria for persistent infection.
