In vitro study of inhibitory effects of pregnancy-induced regulatory T cells on na?ve T cells prolifera-tion
10.3760/cma.j.issn.0254-5101.2013.07.003
- VernacularTitle:妊娠诱导的调节性T细胞对na?ve T细胞抑制作用的体外研究
- Author:
Qin ZHOU
;
Shilong XIANG
;
Qi LI
;
Jieru CAI
;
Hao YANG
;
Hong JIANG
;
Zhangfei SHOU
;
Jianghua CHEN
- Publication Type:Journal Article
- Keywords:
piTreg;
Sorting;
Amplification;
Inhibition
- From:
Chinese Journal of Microbiology and Immunology
2013;(7):495-500
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the in vitro inhibitory effects of regulatory T cells ( Treg ) from unpregnant mice and pregnancy-induced regulatory T cells ( piTreg) on the proliferation of na?ve T cells and their differences .Methods The numbers of piTreg cells from allogeneic pregnant mice ( C57/B6 fe-male×BALB/c male) on day 12.5 (E12.5d) of gestation and Treg cells from unpregnant C57/B6 mice were detected respectively by flow cytometry .The percentages of piTreg cells and Treg cells in CD 4+T cells of age-matched female mice and their intracellular expression of Foxp 3 were analyzed .The in vitro inhibitory effects of piTreg cells and Treg cells on the CFSE-labeled na?ve T cells ( effector cells ) were compared in a one-way mixed lymphocyte culture system using mitomycin C-inactivated CD4-T cells as stimulator cells . Results The level of piTreg cells in splenic mononuclear cells was significantly higher than that of Treg cells (P<0.001) from normal mice.Foxp3 was highly expressed in both piTreg cells and Treg cells , howev-er slightly increased in piTreg cells .Moreover , piTreg cells had a significant stronger in vitro inhibitory effect on na?ve T cells proliferation than that of Tregs cells (P<0.006), which was in a cell-dependent manner. Conclusion The present study suggests that the piTreg cells have a stronger inhibitory effect on na ?ve T cell proliferation as compared with Terg cells from unpregnant mice , The differential activity of CD 4+CD25+Treg might be mediated by the paternal antigens during pregnancy .
