Effects of hIFN-λ1 recombinant adenovirus on proliferation of gastric adenocarcinoma cell line and its mechanism
10.3760/cma.j.issn.0254-5101.2013.07.005
- VernacularTitle:人 IFN-λ1重组腺病毒对胃癌细胞增殖的影响及其机制研究
- Author:
Jun LIU
;
Yulan YAN
;
Jin ZHANG
;
Yang LIU
;
Jie ZHANG
;
Bing LIANG
;
Xuefeng BU
- Publication Type:Journal Article
- Keywords:
Gastric cancer;
IFN-λ;
Recombinant adenovirus;
Apoptosis
- From:Chinese Journal of Microbiology and Immunology
2013;(7):507-512
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of Ad-hIFN-λ1 recombinant adenovirus on prolifera-tion of gastric adenocarcinoma cell line SGC-7901 and its mechanism .Methods Ad-hIFN-λ1 recombinant adenovirus and empty plasmid Ad-LacZ were respeetively transfated to human gastric adenocarcinoma SGC-7901 cells.The proliferation of transfected cells was detected by MTT assay .IFN-λ1 gene expression at mR-NA and protein levels in the cells were measured by RT-PCR analysis and immunofluorescence assay , re-spectively .Tunel assay and flow cytometry were used to analyze the rate of cell apoptosis .Results The proliferation of gastric adenocarcinoma SGC-7901 cells were significantly inhibited with Ad-hIFN-λ1 inter-vention in accordance with highly expressed IFN-λ1 at mRNA and protein levels , respectively .The apoptosis rate of Ad-hIFN-λ1 transfected cells was markedly higher than that of the empty plasmid Ad-LacZ group and PBS blank control group .Conclusion The expression of hIFN-λ1 could be detected after transfection of Ad-hIFN-λ1 recombinant adenovirus into gastric adenocarcinoma SGC-7901 cells.Ad-hIFN-λ1 could signifi-cantly inhibit the proliferation of gastric adenocarcinoma SGC-7901 cells by promoting the apoptosis of cancer cells.
