A comparison of three methods for detecting respiratory viruses
10.3760/cma.j.issn.0254-5101.2013.07.012
- VernacularTitle:三种呼吸道病毒实验室检测方法比较
- Author:
Aihua LI
;
Tiegang ZHANG
;
Weixian SHI
;
Shujuan CUI
;
Meng CHEN
;
Jiang WU
;
Fang HUANG
- Publication Type:Journal Article
- Keywords:
Respiratory virus;
Conventional PCR;
Multiplex RT-PCR;
Multiplex real-time RT-PCR
- From:
Chinese Journal of Microbiology and Immunology
2013;(7):541-544
- CountryChina
- Language:Chinese
-
Abstract:
Objective To screen a sensitive method for detecting respiratory viruses from three different methods of singleplex conventional PCR , multiplex conventional PCR and multiplex real-time RT-PCR.Methods Parallel examination of 17 respiratory viruses was performed on 73 throat swab specimens collected from patients with upper respiratory tract infection by the three methods .The detection rates of dif-ferent respiratory viruses were used as evaluating indicator for the three methods .Results The numbers of respiratory viruses detected by singleplex conventional PCR , multiplex conventional PCR and multiplex real-time PCR were 56, 41 and 87, respectively.Conclusion The multiplex real-time RT-PCR might be used for the detection of respiratory viruses in laboratory as its high detection rate in comparison with the other two methods .
