Screening Effective Components from Fructus Citri Sarcodactylis by Cell Membrane Chromatography and HPLC/MS

VernacularTitle:细胞膜色谱法与液相色谱-质谱联用筛选佛手的活性成分
Author: Yanwei WANG ; Ruimiao CHANG ; Xin WANG ; Yuan YUE ; Hui XUE ; Xiaoni LI
Publication Type:Journal Article
Keywords: cell membrane chromatography; LC/MS; active component; bergapten
From: Chinese Journal of Information on Traditional Chinese Medicine 2013;(8):66-68
CountryChina
Language:Chinese
Abstract: Objective To screen the active component from Fructus Citri Sarcodactylis which have aortic vascular relaxant effect. Methods The active components from Fructus Citri Sarcodactylis were screened by combining vascular smooth muscle/cell membrane chromatography (VSM/CMC) and liquid chromatography/mass spectrometry (LC/MS) with pharmacological assay in vitro. Results Bergapten was the active component of Fructus Citri Sarcodactylis that functions in vascular. The pharmacological experiment showed that bergapten had vasodilating effect. The capacity factor of this compound on the VSM/CMC model was found to be significantly correlated with its pharmacological effect. Conclusion Association between compound and membrane protein (receptor) can be reflected by using the VSM/CMC model. VSM/CMC-offline-LC/MS technology can be used to quickly screening new active components from natural medicine.