Influence of esculentoside A on proliferation and CDK2 of IL-1βinduced glomerular mesangial cell *
10.3969/j.issn.1671-8348.2013.21.021
- VernacularTitle:商陆皂苷甲对IL-1β诱导的肾小球系膜细胞增殖及CDK2、P27的影响
- Author:
Xianggui ZHANG
;
Jieyin TANG
- Publication Type:Journal Article
- Keywords:
mesangial cells;
cell proliferation;
cell cycle;
esculentoside A
- From:
Chongqing Medicine
2013;(21):2496-2499
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of esculemoside A (EsA)on the proliferation and expression of CDK2 and P27 of rats glomerular mesangial cell .Methods rGMC was cultured in vitro ,The cell growth and cell toxicity were detected by MTT assay ;rGMC proliferation was observed by Rnase/PI-Flous ;The expression of CDK2 and P27 were measured by Western blotting .Results EsA at observed dose has not apparent cytotoxicity effect on rGMC .EsA(2 .5-5 .0 mg/L) inhibited the proliferation of rGMC after 48h .EsA increased the number of the G1 phase and reduced the number of the S phase of IL-1βinduced rGMC .At the same time ,EsA inhibited the expression of CDK2 and promoted the expression of P27 of IL-1βinduced rGMC .Conclusion The GMC is one of the mainly target cell which EsA bing therapeu tical action .EsA inhibited proliferation of IL-1βinduced the GMC ,inhibited the expression of CDK2 and activated the expression of P27 may be its mechanism .
