The expression of miR-92b gene in SHG-44 glioma cells and glioma stem cells*
10.3969/j.issn.1671-8348.2013.24.023
- VernacularTitle:SHG-44神经胶质瘤干细胞分化为胶质瘤细胞前、后miR-92b基因的表达变化
- Author:
Xiuqin ZHAN
;
Liudi YUAN
- Publication Type:Journal Article
- Keywords:
miR-92b;
microRNA;
glioma cells;
tumor stem cells
- From:
Chongqing Medicine
2013;(24):2881-2883
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the expression level of miR-92b in SHanG-44 glioma cells and glioma stem cells .Methods The tumor stem cells were screened from the SHG-44 glioma cells and identified by immunofluorescence .Then it was induced to differentiate .The transcription level of miR-92b was determined by RT-PCR and real-time quantitative PCR before and after differ-entiation .Results The miR-92b expression level in differentiated tumor cells was about 3 times higher than in tumor stem cells . Conclusion T he miR-92b gene is involved in the process of differentiation of glioma stem cells .
