Adipose-derived stem cells combined with gelatin sponge for repair of rabbit radial defects
10.3969/j.issn.2095-4344.2013.34.004
- VernacularTitle:脂肪干细胞复合明胶海绵修复兔桡骨缺损
- Author:
Gang LIU
;
Mingxing WANG
;
Changming YAN
;
Yunqing WANG
;
Xuejie WEI
;
Zhaoli HU
- Publication Type:Journal Article
- Keywords:
biomaterials;
tissue-engineered bone materials;
adipose-derived stem cells;
radial defects;
bone morphogenetic protein;
cell culture;
histology;
radiology
- From:
Chinese Journal of Tissue Engineering Research
2013;(34):6083-6088
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Adipose-derived stem cells discovered recently are a new kind of adult stem cells, and have a strong multi-differentiation capacity. However, there are rare studies concerning in vivo osteogenic capacity of adipose-derived stem cells.
OBJECTIVE:To investigate the effect of adipose-derived stem cells combined with gelatin sponge on repairing bone defects.
METHODS:Adipose-derived stem cells from rabbit inguinal fat pads were isolated and cultured, and then
induced using an osteogenic medium containing bone morphogenetic protein 2 fol owed by injection of gelatin sponge. Radial defect models of rabbits were prepared. Compound of adipose-derived stem cel s and gelatin sponge was implanted into the lesion side, while gelatin sponge alone was implanted into the contralateral side. Rabbits were kil ed at weeks 6 and 12 after bone defect repair for X-ray examination, CT scan, and hematoxylin-eosin staining.
RESULTS AND CONCLUSION:Lane-Sandhu X-ray and Lane-Sandhu histological scores after compound implantation were significantly higher than those after repair with gelatin sponge alone. It indicates that
adipose-derived stem cel s combined with gelatin sponge can promote bone defect healing of rabbits, showing an obvious osteogenic capacity in vivo.
