Effect of ABCCA gene silencing on cell proliferation and cell cycle in human pancreatic cancer cell lines PANC1 and BxPC-3
10.3760/cma.j.issn.1674-1935.2013.04.011
- VernacularTitle:沉默ABCC4基因表达对人胰腺癌PANC1、BxPC-3细胞增殖的影响
- Author:
Jin MA
;
Zhuo ZHANG
;
Jiancheng WANG
- Publication Type:Journal Article
- Keywords:
Pancreatic neoplasms;
ABCCA;
Cell,proliferation;
RNA interference;
Lentivirus infections
- From:
Chinese Journal of Pancreatology
2013;13(4):252-255
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the effect of ABCC4 gene silencing on cell proliferation and cell cycle in human pancreatic cancer cell lines PANC1 and BxPC-3.Methods PANC1 and BxPC-3 pancreatic cancer cells were transfected with a lentivirus expressing an ABCCA short hairpin RNA (shRNA).ABCC4 mRNA and protein expression of transfected cells was determined by RT-PCR and Western blot,colony formation ability was measured by colony assay,and cell cycle change was investigated by the flow cytometric analysis.Results Lentivirus expressing an ABCC4 short hairpin RNA (shRNA) was successfully established.After transfection with shRNA lentivirus,ABCC4 mRNA and protein expression were significantly inhibited (0.28 ±0.01 vs 1.00 ±0.03,0.22 ±0.02 vs 1.00 ±0.03,P ＜0.05).Colony formation ability was significantly decreased (4 vs 65,P ＜0.05),and cell cycle was significantly blocked at G1 phase [(54.98 ±1.78) ％ vs (42.93 ± 0.88) ％,(68.55 ± 0.75) ％ vs (54.76 ± 0.29) ％].Conclusions ABCC4 gene silencing can significantly inhibit cell proliferation of human pancreatic cancer cell lines PANC1 and BxPC-3,and block the cells at G1 phase.
