Insulin-like growth factor 1 affects the apoptosis of rat condylar chondrocytes
10.3969/j.issn.2095-4344.2013.033.001
- VernacularTitle:大鼠髁突软骨细胞凋亡与胰岛素样生长因子1的影响*
- Author:
Li WEI
;
Liting JIANG
;
Qi ZHOU
;
Yaping ZHU
;
Yiming GAO
- Publication Type:Journal Article
- Keywords:
tissue construction;
cartilage tissue construction;
insulin-like growth factor 1;
mandibular condyle;
chondrocytes;
cel apoptosis;
cel proliferation;
Bax;
Bcl-2;
provincial grants-supported paper
- From:
Chinese Journal of Tissue Engineering Research
2013;(33):5901-5908
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Insulin-like growth factor 1 is the key factor during cartilage development, which is involved in the growth and reconstruction of condylar cartilage.
OBJECTIVE:To study the effect of insulin-like growth factor 1 on cel apoptosis and the apopotosis-associated factors of Bcl-2, Bax mRNA and protein expressions of rat condylar chondrocytes.
METHODS:The 1-day-old and 28-day-old rat condylar chondrocytes were cultured and identified in vitro. The condylar chondrocytes with different ages were divided into experimental group and control group. After being starved for 24 hours, chondrocytes in the experimental group were incubated with 100μg/L recombined rat insulin-like growth factor 1 for 48 hours, while the chondrocytes in the control group were incubated normal y. RESULTS AND CONCLUSION:Compared with the control group, after being incubated with recombined
insulin-like growth factor 1, the number of condylar chondrocytes was increased with high speed proliferation (P<0.05). Real-time RCR and western blot analysis revealed that the expression levels of Bcl-2 mRNA and protein were increased after added with recombined rat insulin-like growth factor 1, while the expression levels of Bax and protein were decreased (P<0.05). The results indicate that insulin-like growth factor 1 can promote the
proliferation and reduce cel apoptosis of newborn and adolescent rat condylar chondrocytes, which may be mediated by Bcl-2 and Bax.
