Effects of Xuebijing on apoptosis induced by traumatic brain injury in rabbits
10.3760/cma.j.issn.1671-0282.2013.07.011
- VernacularTitle:血必净对兔创伤性脑损伤脑细胞凋亡的影响
- Author:
Jingyan XU
;
Min YAN
;
Gang CHEN
;
Lina YU
- Publication Type:Journal Article
- Keywords:
Xuebijing injection;
Apoptosis;
Brain injury
- From:
Chinese Journal of Emergency Medicine
2013;22(7):741-743
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effects of Xuebijing on apoptosis induced by traumatic brain injury in rabbits.Methods A total of 36 healthy adult male rabbits were randomly devided into 3 groups (n =12):sham group (group S),traumatic brain injury group (group T),and Xuebijing therapy group (group X).The model of traumatic brain injury was produced by improved Feeney' s traumatic brain injury model.Group S drill bone window and not crack down on brain tissue.The rabbits were sacrificed for injury brain tissue samples at 24 h after molding success in every groups.Brain cell apoptosis was evaluated by TUNEL staining.The cerebral expression of Bcl-2 and Bax was analyzed by Western blot.Results Compared with group S,the rates of apoptosis were increased and the protein expression ratios of Bcl-2/Bax were decreased in group T,and group X (P ＜0.01).Compared with group T,the rates of apoptosis were decreased and the protein expression ratios of Bcl-2/Bax were increased in group X (P ＜ 0.01).Conclusions Xuebijing can reduce the apoptosis of cerebral cells induced by traumatic brain injury to a certain extent,and protect the cerebrum,the signal of Bcl-2/Bax change to the way of anti-apoptosis is involved in the mechanism.
