Detection of early brain injury in a piglet model after deep hypothermic circulatory arrest with magnetic resonance imaging
10.3760/cma.j.issn.1001-4497.2013.08.007
- VernacularTitle:磁共振弥散加权成像和组织病理学检测深低温停循环动物术后早期脑损伤
- Author:
Haipeng ZHAO
;
Jie LU
;
Yue TANG
;
Jun ZHENG
;
Xudong PAN
;
Lizhong SUN
- Publication Type:Journal Article
- Keywords:
Cardiopulmonary bypass;
Circulatory arrest,deep hypothermic;
Induced;
Brain injury;
Diffusion magnetic resonance imaging
- From:
Chinese Journal of Thoracic and Cardiovascular Surgery
2013;29(8):470-474
- CountryChina
- Language:Chinese
-
Abstract:
Objective Optimal detection of deep hypothermic circulatory arrest (DHCA)-induced early brain injury is important but the effective technique is still not available in the present.The relationship between diffusion wcighted imaging (DW1) and histopathological changes in DHCA-induced piglet brain injury model were analyzed.Methods Eighteen pigs underwent deep hypothermic circulatory arrest and were divided into three groups:group A (n =6) served as control,only underwent anesthesia and thoracotomy,without extracorporeal circulation.Group B (n =6) served as underwent deep hypothermic circulatory arrest at 20 ℃ for 120 min,survived for 1 day.Group C (n =6) served as underwent deep hypothermic circulatory arrest at 20 ℃ for 120 min,survived for 2 days.Diffusion-weighted imaging and histopathology were used to study the brain injury.Results There were hematoxylin-eosin (+) and terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling (+) cells in cerebral cortex and hippocampus.Hyperintensity was most evident in the cerebral cortex in group B and group C with diffusion-weighted imaging,but it was not seen in hippocampus in both groups.There were 5 and 6 piglets can be seen hyperintensity with diffusion-weighted imaging sequence in group B and group C respectively.Hyperintensity was also seen in T2WI sequence in 3 and 5 piglets in group B and group C respectively.Conclusion The study demonstrates the feasibility of diffusion-weighted imaging on evaluation of brain injury after deep hypothermic circulatory arrest and cerebral cortex was selectively vulnerable to cell injury.It is superior to conventional imaging.
