Effect of photodynamic therapy on the phosphorylation of Smad3 in hyperplastic scar fibroblasts
10.3760/cma.j.issn.0412-4030.2013.06.006
- VernacularTitle:光动力疗法对增生性瘢痕成纤维细胞中Smad3蛋白磷酸化的影响
- Author:
Hong CAI
;
Ying GU
;
Wei LIU
;
Jing ZENG
;
Ning DONG
;
Ping SUN
- Publication Type:Journal Article
- Keywords:
Cicatrix,hypertrophic;
Fibroblasts;
Hematoporphyrin photoradiation;
Smad3 protein
- From:
Chinese Journal of Dermatology
2013;46(6):394-396
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the phosphorylation of Smad3 in hyperplastic scar fibroblasts (HSFs) induced by hematoporphyrin monomerthyl ether (HMME) followed by photodynamic therapy (PDT).Methods Fibroblasts were isolated from the hypertrophic scar tissues of 10 patients and subjected to culture in vitro.After 3-5 passages,the HSFs were divided into 4 groups:control group receiving no treatment,PDT group pretreated with HMME of 4 μg/ml followed by PDT,HMME group induced by HMME alone,and laser group irradiated with laser alone.Fluorescence microscopy was used to observe the expression of Smad3 after immunofluorescent staining with anti-Smad3 antibody,and Western blot to detect the expression of Smad3 and phosphorylated Smad3 in these HSFs.Paired t test was conducted to compare the difference in Smad3 and phosphorylated Smad3 expression between these groups.Results The total fluorescence intensity of Smad3 was similar between these groups,but the intranuclear fluorescence signal was significantly weaker in the PDT group than in the control group.The level of phosphorylated Smad3 was statistically decreased in the PDT group compared with the control group (0.20 ± 0.02 vs.0.92 ± 0.15,P ＜ 0.05),but no significant difference was observed between the HMME group and laser group (P ＞ 0.05).Conclusion PDT may inhibit the proliferation of HSFs via attenuating the phosphorylation of Smad3.
