MicroRNA expression in murine skin at different stages of hair cycle: a preliminary study
10.3760/cma.j.issn.0412-4030.2013.08.010
- VernacularTitle:C57BL/6小鼠不同毛发周期的皮肤组织微RNA表达的初步研究
- Author:
Zhongming LI
;
Wenrong XU
;
Lei WANG
;
Hejian SHI
;
Weixin FAN
- Publication Type:Journal Article
- Keywords:
Hair;
MicroRNAs
- From:
Chinese Journal of Dermatology
2013;46(8):570-573
- CountryChina
- Language:Chinese
-
Abstract:
Objective To screen differentially expressed microRNAs at different stages of hair cycle in a murine model.Methods This study included 30 inbred female C57BL/6 mice (age,6-8 weeks; body weight,15-18 grams).Hair growth cycle was induced in the back skin of C57BL/6 mice by application of wax/rosin followed by depilation under anesthesia witl 1％ chloral hydrate.Three mice were sacrificed by cervical dislocation on day 0,8 and 20 after the induction,and skin tissue was achieved from the same depilated areas parallel to the spine.Total RNAs were extracted from the murine skin and subjected to microarray analysis of microRNA expression.Results Compared with telogen skin,the murine anagen skin showed a higher expression of miR-690,obselote-49 and miR-1308,but a lower expression of miR-291a-5p and miR-212.The expressions of miR-690,obselote-49 and miR-31 were significantly up-regulated,while those of miR-127-3p and miR-212 were downregulated in the catagen skin in comparison with the telogen skin.Conclusion Seven microRNAs are identified in this study to be differentially expressed in murine skin between different stages of hair cycle,which may provide a direction for future research.
