Assessment of muscle perfusion in ischemic rabbit hindlimb treated by intravenous implantation of bone marrow mesenchymal stem cells with perfusion CT
10.3760/cma.j.issn.1005-1201.2013.08.019
- VernacularTitle:CT灌注成像评价兔缺血后肢干细胞移植后血管生成情况的研究
- Author:
Fei HUANG
;
Zaiming LU
;
Hui WANG
;
Guoqiang REN
;
Xiaomei LU
- Publication Type:Journal Article
- Keywords:
Tomography,X-ray computed;
Mesenchymal stem cells;
Transplantation;
Neovascularization,physiologic
- From:
Chinese Journal of Radiology
2013;47(8):745-748
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the application of CT perfusion in the assessment of muscle perfusion in ischemic rabbit hindlimb treated by implantation of bone marrow mesenchymal stem cells.Methods Twenty rabbits were divided into two groups randomly (each n =10).Bone marrow mesenchymal stem cells were injected into the left ischemic hind limbs of animal models of intravenous injection group,with same volume of normal saline injected into the control group.Twenty-eight days later,the collateral circulation and blood vessel neogenesis were examined by Toshiba Aquilion One 320-MSCT and pathological approaches.t test and Pearson test were used to compare the parameters.Results The results show that rAF,rBV,rClearance,rMVD were 1.15 ±0.67,1.19 ±0.32,0.62 ±0.20,and 1.34 ±0.28 in intravenous injection group and 0.57 ±0.17,0.74 ±0.19,2.06 ±0.15,0.62 ±0.19 in the control group respectively.There was significant difference of rAF,rBV,rClearance and rMVD between the intravenous injection group and the control group(t =5.75,5.01,-2.81,6.43 respectively,P ＜ 0.01).There was significant correlation between rBV,rAF,rC and rMVD (r =0.857,0.811,0.615,P ＜ 0.01).Conclusion CT perfusion imaging is a relatively accurate technique to assess changes of muscle perfusion in ischemic rabbit hindlimb treated by implantation of bone marrow mesenchymal stem cells.
