Enhanced gene transfection to HepG2 cells mediated by self-made cationic nanobubbles

VernacularTitle:自制阳离子纳米泡介导HepG2细胞基因转染的实验研究
Author: Xiaoling LIU ; Yunchao CHEN ; Lu DING ; Naxiang LIU ; Qingping ZHANG
Publication Type:Journal Article
Keywords: Ultrasonography; Microbubbles; Nanotechnology; Polyethyleneimine; Transfection
From: Chinese Journal of Ultrasonography 2013;22(7):621-625
CountryChina
Language:Chinese
Abstract: Objective To observe self-made cationic nanobubbles as non-viral gene carrier to transfer green fluorescent protein reporter gene into HepG2 cell in vitro.Methods Cationic nanobubbles(PNB) were prepared by sonicating liposomes、polyethylenimine and perfluoropropan.The surface potential and the size of nanobubbles were assessed by laser particle analyzer.HepG2 cells were incubated with DNA,nanobubbles with or without ultrasound exposure.The transfection efficiency was evaluated by flow cytometer and the cell viability by cell counting Kit-8.Results The mean diameter of PNB was (834.57 ± 6.4) nm and the surface charge was (4.15± 0.98)mV.The PNB-DNA complexes,which blocked by the Agarose gel electrophoresis,could effectively transfer HepG2 cells,and the ultrasound exposure could enhance the transfection efficiency further significantly (P ＜ 0.05).Conclusions The new PNB could effectively combine with pDNA to enhance gene delivery and ultrasound exposure could improve its efficiency further in HepG2 cell in vitro.