Effects of l-tetrahydropalmatine on conditioned place preference and expression of dopamine in the striatum of morphine dependent rats
10.3760/cma.j.issn.1674-6554.2013.07.005
- VernacularTitle:左旋延胡索乙素对大鼠吗啡条件性位置偏爱效应及纹状体多巴胺系统的影响
- Author:
Weifeng BAI
;
Peirun YANG
;
Shouyang YU
;
Ping TU
;
Mingsong WU
;
Gang QIAN
;
Suyuan LUO
- Publication Type:Journal Article
- Keywords:
l-tetrahydropalmatine;
Morphine;
Psychological dependence;
Dopamine system
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2013;22(7):591-593
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe effects of conditioned place preference (CPP) of morphine dependent rats,variation of dopamine neurotransmitter and its receptor 2 of the striatum in rats suffered from 1-tetrabydropalmatine(l-THP).Methods The CPP model was established by morphine injection in rats with a increasing dose for 10 days,with the initial dose of 10 mg · kg-1 and the final dose of 100 mg · kg-1,10 mg · kg-1 was increased each day,thus 100 nmg · kg-1 Was injected by day 10.Treatments with administration of 1-THP(3.76,1.88 and 0.94 mg/kg) were performed respectively for 6 days,and the effects of CPP for psychological dependence in these rats were observed.Striatum samples were taken out and their variable contents of dopamine neurotransmitter and its receptor 2 in striatum were detected by high performance liquid chromatography and immunohistochemisty,respectively.Results Compared with the NS treatment group,the time of animals treated with 1-THP (3.76 and 1.88 mg/kg) staying in drug-paired compartment were reduced (354 ± 58,373 ± 79) (P＜ 0.01) ;the raised of variation contents of dopamine neurotransmitter were reduced in striatum from those rats(5.49 ± 1.95,6.11 ± 1.05),while the expression level of dopamine receptor 2 was increased(0.08 ± 0.02,0.07 ± 0.03) (P ＜ 0.01 or P ＜0.05).Conclusion Reversing dopamine neurotransmitter and its receptor 2 in striatum of morphine dependent rats may be one of the possible mechanisms that 1-THP effectively inhibit the effects of morphine CPP.
