Explore the protective effects of the bone marrow mesenchymal stem cells on severe acute pancreatitis in rats
10.3760/cma.j.issn.1671-0282.2013.04.012
- VernacularTitle:骨髓间充质干细胞对大鼠重症急性胰腺炎损伤修复的作用
- Author:
Yuqiang SHAN
;
Liang LUO
;
Zhong JIA
;
Yang CAI
;
Huicheng JIN
;
Guanghua FENG
- Publication Type:Journal Article
- Keywords:
Mesenchymal stem cells;
Severe acute pancreatitis;
Protective effects;
Granulocyte colony-stimulating factor
- From:
Chinese Journal of Emergency Medicine
2013;22(4):395-399
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate effects of bone marrow mesenchymal stem cells (MSC) on severe acute pancreatitis (SAP) in rats.Methods A total of 60 rats were randomly divided into normal group (n =10),the model of SAP group (n =10),the transplantation of the MSC group (n =20),the combination of the MSC and the granulocyte colony-stimulating factor (G-CSF) group (n =20).The conversion rate of MSC was detected by using immunofluorescence methods,and the level of amylase in serum was assayed by using biochemical methods.Simultaneously,the level of interleukin-6 (IL-6) in serum was determined by using enzyme linked immunosorbent assay (ELISA).Results The conversion rates of the MSC increased,and consequently,the levels of amylases and interleukin-6 in serum were reduced (P ＜ 0.05).When a small amount of the G-CSF was added to MSC,the therapeutic effects of the two different kinds of cells were synergistically strengthened.In the contrary,when a large number of the G-CSF was added to MSC,the antagonism resulted between these two different kinds of cells gives rise to harmful effects on SAP.Conclusions The bone marrow mesenchymal stem cells have therapeutic effects on SAP.When the number of the bone marrow mesenchymal stem cells increases,the protective effects are enhanced.
