Histocompatibility of adipose-derived stem cells and absorbable gelatin sponge
10.3969/j.issn.2095-4344.2013.40.005
- VernacularTitle:脂肪干细胞与可吸收明胶海绵的组织相容性
- Author:
Wei ZHANG
;
Yuanhe ZHANG
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2013;(40):7047-7053
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:It is particularly important to search favorable scaffolds and seed cells in bone tissue engineering. However, no existing studies have reported the co-culture of adipose-derived stem cells and absorbable gelatin sponge.
OBJECTIVE:To induce rabbit adipose-derived stem cells to osteoblasts, observe the adhesion, proliferation and biological characteristics of adipose-derived stem cells on absorbable gelatin sponge, and to provide experimental base for absorbable gelatin sponge as an effective carrier of fat stem cells transplantation in vivo.
METHODS:Adipose-derived stem cells were cultured in vitro and identified with immunohistochemistry and flow cytometry. The passage 3 adipose-derived stem cells were induced to differentiate into osteoblasts and then seeded onto absorbable gelatin sponge after treatment with polylysine. The adhesion and proliferation of adipose-derived stem cells on scaffolds were observed under scanning electron microscope.
RESULTS AND CONCLUSION:The rabbit adipose-derived stem cells were mainly round and oval shaped at 48 hours, and became long spindle shaped after 48 hours. Adipose-derived stem cells were positive for CD29 and CD44, and negative for CD33 and CD34. Adipose-derived stem cells can be induced to differentiate into osteoblasts after culture in the osteogenesis induced fluid for 72 hours. The induced osteoblasts that were co-cultured with absorbable gelatin sponge had an 85%adherence rate at 24 hours. The cells began to extend pseudopodium at 48 hours, and osteoblasts adhered in clumps and secreted a large amount of cellmatrix at 7 days. Absorbable gelatin sponge began to absorb and degrade. Experimental findings indicate that, adipose-derived stem cells have good histocompatibility with absorbable gelatin sponge, and absorbable gelatin sponge can be used as a biological carrier of adipose-derived stem cells.
