Rhein and archen induce the apoptosis of mesangial cells in high glucose rats
10.3969/j.issn.2095-4344.2013.44.014
- VernacularTitle:大黄酸及大黄素诱导高糖大鼠肾小球系膜细胞的凋亡
- Author:
Yu GUO
;
Yan LI
;
Hui FANG
;
Xiaoxing YIN
;
Qunli WEI
- Publication Type:Journal Article
- Keywords:
organ transplantation;
kidney transplantation;
kidney glomerulus;
diabetic nephropathies;
apoptosis
- From:
Chinese Journal of Tissue Engineering Research
2013;(44):7739-7744
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Our previous study has already manifested that Chinese medicine Xiaokening can effectively prevent and treat the early diabetic nephropathy. OBJECTIVE:To compare the effect of rhein and archen on the apoptosis of mesangial cells of rats cultured with high glucose. METHODS:Mesangial cells were stimulated by different concentrations of rhein and archen (20, 40 and 80 μmol/L). Morphology of apoptotic cells was observed by hematoxylin-eosin staining. Karyon apoptosis was examined by fluorescence staining of DAPI. cellapoptosis rate was detected by flow cytometry. RESULTS AND CONCLUSION:The outcome of hematoxylin-eosin staining and DAPI staining indicated that the effect of rhein was much stronger than that of archen on the apoptosis of mesangial cells in the rats cultured with high glucose. Comparison of the apoptosis rate also indicated that the rhein had a stronger effect on the earlier and the later stage apoptotic rate of mesangial cells than archen. Both rhein and archen with different concentrations can induce apoptosis of mesangial cells, but the effect of rhein is much stronger.
