Result analysis of four human leukocyte antigen rare alleles
10.3969/j.issn.2095-4344.2013.44.025
- VernacularTitle:4例人类白细胞抗原罕见等位基因的结果分析
- Author:
Daming WANG
;
Liumei HE
;
Hongyan ZOU
;
Suqing GAO
- Publication Type:Journal Article
- Keywords:
HLA antigens;
alleles;
bone marrow;
organ transplantation;
cell transplantation
- From:
Chinese Journal of Tissue Engineering Research
2013;(44):7809-7814
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Many articles concerned novel al eles reported in China and outside China, but some al eles were detected lately. After that, these al eles were tested again. Because frequencies of these al eles are very low, few relevant articles are reported, so these al eles are ignored easily. OBJECTIVE:To test and identify four human leukocyte antigen rare al eles that patients and donors carries. METHODS:Genomic DNA was extracted automatical y from blood samples using quick DNA purified kit, typed by human leukocyte antigen locus commercial sequence-based typing kits and confirmed by sequence-specific primers high-resolution kits. RESULTS AND CONCLUSION:Four detected rare al eles are B*27:15, B*51:39, C*07:66 and C*08:22. The four above-mentioned human leukocyte antigen rare al eles defined by National Marrow Donor Program are not rare in China. The facts prove that C*08:22 which was defined a rare al ele by National Marrow Donor Program before is a common al ele in Chinese Han nationality.
