Silenced estrogen receptor beta affects the expressions of osteoprotegerin and receptor activator of nuclear factor-kappa B ligand in osteoblastic MG63 cells
10.3969/j.issn.2095-4344.2013.41.002
- VernacularTitle:雌激素受体β基因沉默对成骨样MG63细胞骨保护素和RANKL表达的影响
- Author:
Yuxiang WANG
;
Hongqi ZHANG
;
Chaofeng GUO
;
Mingxing TANG
;
Shaohua LIU
;
Ang DENG
;
Qile GAO
;
Zhansheng DENG
;
Jing CHEN
;
Jinyang LIU
;
Jianhuang WU
- Publication Type:Journal Article
- Keywords:
estrogen receptor beta;
osteoprotegrin;
receptor activator of nuclear factor kappa B;
osteoblasts
- From:
Chinese Journal of Tissue Engineering Research
2013;(41):7188-7198
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Studies concerning how estrogen receptorβparticipates in bone metabolism are few now. OBJECTIVE:To investigate the effect of estrogen receptorβon the expression of osteoprotegerin and receptor activator of nuclear factor-κB ligand in human osteblast-like cells. METHODS:The retrovirus with the most effective interference sequence and non-specific short hairpin RNA was used to transfect human osteoblast-like cellMG63 in order to screen out the stable colon, and then amplified and cultured. The blank control and non-specific short hairpin RNA were used as control, and the stable inhibition rate of estrogen receptorβwas detected. The 17β-estradiol was added into the cells in three groups, that were MG63 cells, short hairpin RNA retrovirus estrogen receptorβ-mediated MG63 cells and negative control short hairpin RNA retrovirus-medicated MG63 cells, in order to detect the expressions of osteoprotegerin and receptor activator of nuclear factor-κB ligand mRNA in human osteoblast-like cells. RESULTS AND CONCLUSION: The human osteoblast-like MG63 cellline was further stably transfected with pRNAT-H1.4/Retro-estrogen receptorβshort hairpin RNA3, and then compared with the blank control and negative control, and found that estrogen receptorβcould express the stable inhibited human osteoblast-like cellline. The inhibition rate of estrogen receptorβmRNA was (88.17±1.17)%(P<0.05), and the inhibition rate of estrogen receptorβprotein was (89.01±1.22)%(P<0.05), indicating that estrogen receptorβgene knockdown human osteoblast-like cellmodels were constructed successful y. After estrogen intervention for 48 hours, the inhibition of MG63 cells with estrogen receptorβcould up-regulate the osteoprotegerin mRNA and protein expression in the blank control group and the negative control group (P<0.05), down-regulate the receptor activator of nuclear factor-κB ligand mRNA and protein expression (P<0.05), and up-regulate the osteoprotegerin receptor activator of nuclear factor-κB ligand expression. The results indicate that estrogen receptorβmay play an important role in bone metabolism through regulating osteoprotegerin/receptor activator of nuclear factor-κB ligand ratio.
