Biological characteristics of synovial mesenchymal stem cells after isolation and purification
10.3969/j.issn.2095-4344.2013.41.006
- VernacularTitle:滑膜间充质干细胞分离纯化后的生物学特性
- Author:
Peiliang FU
;
Lei ZHANG
;
Yuli WU
;
Haishan WU
;
Ruijun CONG
;
Song CHEN
;
Zheru DING
;
Qi ZHOU
- Publication Type:Journal Article
- Keywords:
synovial membrane;
mesenchymal stem cells;
menisci,tibial;
karyotyping;
carcinogenicity tests
- From:
Chinese Journal of Tissue Engineering Research
2013;(41):7221-7227
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:A large amount of studies have confirmed that synovial mesenchymal stem cells have the similarity in cellmorphology, immune phenotype, colony forming ability and differentiation potential with bone marrow mesenchymal stem cells. But bone marrow mesenchymal stem cells are better than synovial mesenchymal stem cells in the ability to differentiate into cartilages. OBJECTIVE:To discuss the possibility of using synovial mesenchymal stem cells as seed cells for meniscal tissue engineering. METHODS:The synovial mesenchymal stem cells were isolated from rabbit synovial tissues with limiting dilution monoclonal culture method, and then the cells were purified. The morphology, ultrastructure, molecular phenotype, proliferation kinetics, karyotype and tumorigenicity of the in vitro cultured cells were analyzed. RESULTS AND CONCLUSION:The synovial mesenchymal stem cells isolated from the rabbit synovial cells had high proliferation capacity during in vitro monolayer culture. The synovial mesenchymal stem cells grew to peak at 6 days, and the doubling time was (30.2±2.4) hours. Flow cytometry results showed the synovial mesenchymal stem cells could express some molecular makers of mesenchymal stem cells, such as CD44 and CD90. DNA contents check, karyotype test and oncogenicity test confirmed isolated and purified synovial mesenchymal stem cells were the normal diploid cells without tumorigenicity, so the cells can be used as seed cells for meniscal tissue engineering.
