Compatibility of polyurethane membranes after surface modification with human hypopharyngeal fibroblasts
10.3969/j.issn.2095-4344.2013.34.011
- VernacularTitle:表面改性聚氨酯平面膜与人下咽成纤维细胞的相容性
- Author:
Cheng KANG
;
Zhisen SHEN
;
Jingjing CHEN
;
Yabin ZHU
- Publication Type:Journal Article
- Keywords:
biomaterials;
material biocompatibility;
polyurethane;
protein graft;
silk fibroin;
glutin;
human hypopharyngeal fibroblast;
tissue engineering
- From:
Chinese Journal of Tissue Engineering Research
2013;(34):6131-6137
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Polyurethane has good mechanical and physical characteristics and is extensively used in
clinical and experimental studies, but its hydrophobicity and histocompatibility are not ideal, which limits its use in tissue engineering as a biomaterial scaffold to some extents.
OBJECTIVE:To observe the hydrophilicity of polyurethane membrane grafted with silk fibroin and glutin and its compatibility with human hypopharyngeal cel s.
METHODS:The changes in hydrophilicity of polyurethane membrane grafted with silk fibroin and glutin were detected by contact angle measurements. Human hypopharyngeal fibroblasts were cultured in vitro on
polyurethane membrane, silk fibroin-polyurethane membrane, glutin-polyurethane membrane and tissue culture plate. Cel compatibility was compared using cytometry and cel morphology obsevation.
RESULTS AND CONCLUSION:Hydrophilicity of silk fibroin-or glutin-polyurethane membranes significantly increased (P<0.01). The hydrophilicity of silk fibroin-polyurethane membrane was higher than that of
glutin-polyurethane membrane (P<0.01). The number of cel s on the tissue culture plate was the most. The number of human hypopharyngeal fibroblasts on the silk fibroin-or glutin-polyurethane membrane was higher
than that on the polyurethane membrane, especial y on the silk fibroin-polyurethane membrane. These suggested that hydrophilicity and cel compatibility of silk fibroin-or glutin-polyurethane membrane were elevated.
