Expression of matrix metalloproteinases in the synovium of rat knee joint during long-distance running
10.3969/j.issn.2095-4344.2013.33.002
- VernacularTitle:长距离跑步大鼠膝关节滑膜基质金属蛋白酶的表达***
- Author:
Yaxian SHANGGUAN
;
Xiaochun WEI
;
Kai LI
;
Jianping WEI
- Publication Type:Journal Article
- Keywords:
tissue construction;
cartilage tissue construction;
synovitis;
knee joint;
synovial injury;
matrix metal oproteinase;
long-distance;
running exercise;
immunohistochemistry;
National Natural Science Foundation of China
- From:
Chinese Journal of Tissue Engineering Research
2013;(33):5909-5916
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Many studies have shown that matrix metal oproteinases 1, 3, 9 and 13 play an important role in articular cartilage degeneration and destruction, but there is less special research on the articular synovium. OBJECTIVE:To observe the effect of long-distance running on the expressions of matrix metal oproteinases 1, 3, 9 and 13 in the synovium.
METHODS:Fifteen male Wistar rats were divided into three groups:control group, tablet group and uphil group. Rats in the control group received ordinary captivity;rats in the tablet group ran on the horizontal treadmil (0°) at the speed of 1 km/h for 1 hour daily, and lasted for 45 days;rats in the uphil group daily ran on the horizontal
treadmil (0°) at the speed of 1 km/h for 1 hour, and lasted for 15 days, and then the rats ran on the uphil treadmil (+20°) at the speed of 1 km/h for 1 hour daily and lasted for 30 days. The knee joint synovium injury models with varying degrees were established. The dual hind knee joints were obtained after modeling for paraffin-embedded. Then the overal sagittal slices were obtained for hematoxylin-eosin staining and immunohistochemical staining, and the experimental results were observed and analyzed.
RESULTS AND CONCLUSION:After long-distance running, the expression of matrix metal oproteinases 1 in synovium of the tablet group and uphil group was increased when compared with that of the control group (P<0.05), but there was no significant difference between tablet group and uphil group (P>0.05). There was no significant difference in matrix metal oproteinases 3 expression (P>0.05). The expressions of matrix metal oproteinase 9 and
matrix metal oproteinase 13 in synovium were in gradient increasing state (P<0.05), which were lowest in the control group, increased in the tablet group and highest in the uphil group. The results indicate that long-distance running
exercise can influence the normal physiological structure of rat knee joint synovium by changing the expression of matrix metal oproteinases.
