Tougu Xiaotong capsule promotes the proliferation and differentiation of osteoblasts
10.3969/j.issn.2095-4344.2013.33.004
- VernacularTitle:透骨消痛胶囊促进成骨细胞的增殖和分化****
- Author:
Yunmei HUANG
;
Wenlie CHEN
;
Ruhui LIN
;
Meiya HUANG
;
Zuanfang LI
;
Naishun LIAO
;
Xianxiang LIU
- Publication Type:Journal Article
- Keywords:
tissue construction;
bone tissue construction;
Tougu Xiaotong capsule;
osteoarthritis;
subchondral bone;
osteoblasts;
proliferation;
osteoprotegerin/receptor activator of nuclear factorκB ligand;
bone remodeling;
provincial grants-supported paper
- From:
Chinese Journal of Tissue Engineering Research
2013;(33):5923-5928
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Tougu Xiaotong capsule is the clinical prescription for the treatment of osteoarthritis in Fujian University of Traditional Chinese Medicine, and the previous studies mainly focus on effect to cartilage.
OBJECTIVE:To observe the effect of Tougu Xiaotong capsule on the proliferation and differentiation of osteoblasts as wel as the expressions of bone remodeling correlated factors.
METHODS:Rat osteoblast-like cel line ROS17/2.8 cel s were incubated with Tougu Xiaotong capsule. The ROS17/2.8 cel s were divided into blank control group and Tougu Xiaotong capsule groups with different
concentrations. The cel proliferation was determined by methylthiazolyldiphenyl-tetrazolium bromide assay. Osteoblast differentiation biomarkers alkaline phosphatase activity, osteocalcin and bone mineralized nodules were measured with colorimetry, enzyme-linked immunosorbent assay and alizarin red staining, respectively. The real-time PCR and enzyme-linked immunosorbent assay were used to detect the expressions of bone remodeling factors osteoprotegerin/receptor activator of nuclear factorκB ligand.
RESULTS AND CONCLUSION:Compared with the control group, the Tougu Xiaotong capsule with the
concentration of 0.25-2 g/L could significantly promote the ROS17/2.8 cel proliferation (P<0.05), up-regulate the alkaline phosphatase activity, osteocalcin expression level and mineralized nodules area, and increase the
percentage of bone remodeling factors osteoprotegerin/receptor activator of nuclear factorκB ligand (P<0.05). The mechanism of Tougu Xiaotong capsule protecting osteoarthritis may partly result from the regulation of
proliferation and differentiation of osteoblasts and bone remodeling.
