Streptococcus mutans gcp gene knockout strains expression profile gene chip
10.3969/j.issn.2095-4344.2013.33.014
- VernacularTitle:变形链球菌gcp基因敲除菌株表达谱基因芯片*☆
- Author:
Miaomiao XIE
;
Xiaocong HU
;
Buling WU
;
Wenjuan YAN
- Publication Type:Journal Article
- Keywords:
tissue construction;
cytology experiment in tissue construction;
Streptococcus mutans;
gene chip;
c-di-GMP signal pathway;
ahpC;
phosphotransferase system;
National Natural Science Foundation of China
- From:
Chinese Journal of Tissue Engineering Research
2013;(33):5988-5994
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Previous studies have confirmed the presence of bis-(3'-5')-cyclic dimeric guanosine
monophosphate signaling pathway in Streptococcus mutans, which construct the streptococcus mutans gcp gene knockout strains.
OBJECTIVE:To compare the gene expression differences between Streptococcus mutans wild strains and gcp mutant strains, and to screen the biofilm-related genes from them for the fol ow-up study.
METHODS:The total RNA of two kinds of strains were extracted and stained with cy3 and cy5 respectively after reverse transcription. The gene chip was scanned after hybridization and the differential gene were obtained
through the data analysis. The different expression genes were verified by real-time PCR.
RESULTS AND CONCLUSION:Differential genes were mainly relative about glucose metabolism and biofilm formation. We selected two genes for real-time PCR verification. The PCR results were consistent with the
microarray results. After Streptococcus mutans gcp gene knockout, the gene expressions of gcp mutant strains were upregulated and the gene expressions of phosphotransferase system were downregulated, this result
suggested that two different genes were related with the c-di-GMP signal pathway downstream.
