Cytotoxicity of dehydrated ostrich acellular corneal stroma as a carrier material
10.3969/j.issn.2095-4344.2013.33.015
- VernacularTitle:干燥脱水保存鸵鸟脱细胞角膜基质载体材料的细胞毒性*
- Author:
Xianning LIU
;
Xiuping ZHU
;
Jie WU
;
Lifang WANG
;
Yong YIN
- Publication Type:Journal Article
- Keywords:
tissue construction;
cytology experiment in tissue construction;
tissue-engineered cornea;
cornea;
ostrich;
acel ular corneal stroma;
scaffold;
dehydration;
preservation;
cytotoxicity test;
provincial grants-supported paper
- From:
Chinese Journal of Tissue Engineering Research
2013;(33):5995-6000
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Previous studies from Shaanxi Institute of Ophthalmology have shown that ostrich cornea has the advantages to be developed into the alternatives of human corneal material.
OBJECTIVE:To determine the potential toxic effects of ostrich corneal stromal scaffold on cel s.
METHODS:Cel culture methods were used to culture L-929 cel s in the extracts of ostrich acel ular corneal
stroma which was dried and dehydrated. 3-(4,5)-Dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide assay was used to evaluate the growth and proliferation of cel s after cultured for 1, 2 and 3 days.
RESULTS AND CONCLUSION:After the cel s were cultured in the extracts of ostrich acel ular corneal stroma subjected to dryness and dehydration for 1, 3 and 5 days, and the toxicity level of cultured cel s was graded as level 1. The cytotoxicity test was conducted according to the“National Standard of the People's Republic of China GB/T16886.5-2003”. After cultured in the extracts of ostrich acel ular corneal stroma, a smal number of cel s were round in shape and loosely adherent without intracytoplasmic granules, and cel lysis could be observed
occasional y. The results of 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide assay showed that
the ostrich acel ular corneal stromal scaffold which was dried and dehydrated had level 1 of cytotoxicity and could be considered as a qualified material.
