Evaluation of bone marrow mesenchymal stem cells for the treatment of osteonecrosis of femoral head
10.3969/j.issn.2095-4344.2013.35.017
- VernacularTitle:骨髓间充质干细胞治疗股骨头坏死的评价*
- Author:
Ruiqi LI
;
Guoping ZHANG
;
Lizhong REN
;
Yali LI
;
Yajun Lü
- Publication Type:Journal Article
- Keywords:
bone and joint implants;
review of bone and joint;
bone marrow mesenchymal stem cel s;
osteonecrosis of femoral head;
cel culture;
cel tracing;
cel differentiation;
provincial grants-supported paper
- From:
Chinese Journal of Tissue Engineering Research
2013;(35):6327-6332
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:There are various methods for the treatment of osteonecrosis of femoral head, but there is no satisfactory method to promote the repair of osteonecrosis of femoral head. In recent years, bone marrow
mesenchymal stem cel transplantation for the treatment of osteonecrosis of femoral head has achieved certain effect.
OBJECTIVE:To review the application progress and problems of bone marrow mesenchymal stem cel transplantation for the treatment of osteonecrosis of femoral head.
METHODS:A computer-based online search was performed in PubMed database, Wanfang database and CNKI database for the related articles from 1999 to 2012. The articles on the isolation, culture, differentiation, labeling and in vivo tracing of bone marrow mesenchymal stem cel s were selected, as wel as the basic and clinical
researches on bone marrow mesenchymal stem cel transplantation for the treatment of osteonecrosis of femoral head. A total of 39 articles were included for review.
RESUTLS AND CONCLUSION:At present, the method for the isolation of bone marrow mesenchymal stem cel s includes adherence screening method, density gradient centrifugation, flow cytometry separation and magnetic activated cel sorting method;the commonly used method for cel labeling and tracing includes isotope tracing method, antigen labeling method, antigen labeling, fluorescent labeling and MRI contrast enhancer labeling
method. The method for the treatment of osteonecrosis of femoral head with bone marrow mesenchymal stem cel s includes pith dril ing decompression combined with bone marrow mesenchymal stem cel injection and
transplantation, intervention plus bone marrow mesenchymal stem cel transplantation, gene transfection combined with bone marrow mesenchymal stem cel transplantation and tissue engineering technology of bone marrow mesenchymal stem cel s. Although, the research on the bone marrow mesenchymal stem cel transplantation for the treatment of
osteonecrosis of femoral head has achieved great progress, there are stil problems needed to be further solved.
