Biology effect research of dihydroartemisinin induce the apoptosis in PC-3 cell lines of prostate cancer enhanced by low frequency ultrasound
10.3969/j.issn.1671-8348.2013.23.004
- VernacularTitle:低频超声增强双氢青蒿素诱导前列腺癌 PC-3细胞凋亡的生物学效应研究
- Author:
Jing WANG
;
Ziguo LUO
;
Jian LI
- Publication Type:Journal Article
- Keywords:
low frequency ultrasound;
dihydroartemisinin;
prostate cancer;
apoptosis
- From:
Chongqing Medicine
2013;(23):2706-2709
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the enhancement effects of the low frequency ultrasound to the apoptosis induced by dihydroar-temisinin in the transplantation human prostate cancer PC-3 cells in nude mice ,and to explore the underlying action mechanism . Methods Prostate cancer PC-3 cells were transplanted into 20 nude mice to establish the solid tumor mode .These nude mice were randomly divided into 4 groups with 5 mice in each group :control group ,dihydroartemisinin group ,ultrasound group and ultrasound+ dihydroartemisinin group .After 13 days and 7 times drug administration ,The positive products of Bcl-2 ,Bax ,VEGF ,Caspase-3 and CHOP were tested by immunohistochemical method .Results The tumor volume were larger in control group and ultrasound group through observed by tumor contour ,and there were liquefication and necrosis region after incised the tumor tissue .Immuno-histochemical examination revealed that the positive products of Bcl-2 and VEGF were decreased in ultrasound+DHA group ,DHA group and ultrasound group .Immunohistochemical examination revealed that the positive products of Bax 、Caspase-3 and CHOP were significantly increased in ultrasound+DHA group ,DHA group and ultrasound group .Conclusion The low frequency ultra-sound can′t cause obvious damage to the normal cell ,which could causing the change of the positive products in transplantation hu-man prostate cancer PC-3 cells .The results show that DHA has this action alone ,the ultrasound may enhance the apoptosis of PC-3 cells induced by DHA.
