Chloroquine promotes DDP-induced apoptosis in human gastric cancer cell line SGC7901
10.3969/j.issn.1000-8179.20130507
- VernacularTitle:氯喹促进顺铂诱导胃癌SGC7901细胞凋亡及其机制*
- Author:
Huiqing ZHANG
;
Nian FANG
;
Shan LU
;
Bo HE
;
Yiye WAN
- Publication Type:Journal Article
- Keywords:
autophagy;
cisplatin;
chloroquine;
apoptosis;
gastric cancer
- From:
Chinese Journal of Clinical Oncology
2013;(16):947-950
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the mechanism and effects of autophagy on cisplatin(DDP)-induced apoptosis in human gas-tric cancer cell line SGC7901. Methods:Cell proliferation was determined by an MTT assay after the SGC7901 cells were treated with DDP and/or chloroquine. Cell apoptosis was determined by flow cytometry. Autophagy and related protein expressions were detected by Western blot. Autophagy was quantitatively analyzed by fluorescence microscopy after monodansylcadaverine staining was per-formed. Results:The cells were treated with 5 mg/L of DDP for 24 h, the rate of cell apoptosis was (21.07 ± 2.12)%. Autophagy, char-acterized by an increase in the number of autophagic vesicles and LC3-II protein level, was observed in DDP-treated cells. After autoph-agy was inhibited by chloroquine, the rate of cell apoptosis was increased to (30.16 ± 3.54)%. In addition, caspase-3 and P53 protein levels were increased, but Bcl-2 protein was decreased. Conclusion:Autophagy protected human gastric cancer cell line SGC7901 from DDP-induced apoptosis. In addition, the inhibition of autophagy could promote apoptosis. The combined therapy of DDP and chlo-roquine may be a promising therapeutic strategy for gastric cancer.
