Effect of EphrinA1-Fc on phosphorylation of EphA2 and ERK in 786-O renal carcinoma cells
10.3969/j.issn.1000-8179.20130415
- VernacularTitle:EphrinA1-Fc对人肾透明细胞癌786-O细胞EphA2和ERK表达影响的研究*
- Author:
Jinsheng XU
;
Yaling BAI
;
Junxia ZHANG
;
Liwen CUI
;
Huiran ZHANG
;
Shenglei ZHANG
- Publication Type:Journal Article
- Keywords:
EphrinA1-Fc;
786-O renal carcinoma cells;
EphA2;
ERK;
phosphorylation
- From:
Chinese Journal of Clinical Oncology
2013;(16):956-959
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To detect the effect of EphrinA1-Fc on the phosphorylation of EphA2 and extracellular signal-regulated ki-nase (ERK) in 786-O renal carcinoma cells (RCCs). Methods:The soluble ligand EphrinA1-Fc was used to inhibit the 786-O RCCs in vitro. Western blot analysis was used to examine the phosphorylation of EphA2 and ERK1/2 in the 786-O RCCs at different time points. Results:After the intervention with EphrinA1-Fc for 5, 10, 30, and 60 min, the expression of p-EphA2 increased (F=9.392, P=0.025) as well as that of p-ERK (F=4.428, P=0.041). No p-EphA2 and p-ERK expression was observed in the pre-intervention group. Conclusion:One of the possible mechanisms of the inhibitory effect of EphrinA1-Fc on tumor metastasis and recurrence involves the phosphorylation of EphA2 by EphrinA1-Fc, leading to the degradation of EphA2.
