Effects of Naotaifang on Expression of MMP-9, NF-κB and TIMP-1 after Focal Cerebral Ischemia in Rats
10.3969/j.issn.1005-5304.2013.09.010
- VernacularTitle:脑泰方对局灶性脑缺血大鼠脑组织核因子-κB、基质金属蛋白酶9及其抑制剂表达的影响
- Author:
Jun LIAO
;
Wei ZHANG
;
Xing XIA
;
Yongmei SHI
;
An CHEN
;
Jinwen GE
- Publication Type:Journal Article
- Keywords:
Naotaifang;
regional cerebral ischemia;
MMP-9;
NF-κB;
TIMP1;
rats
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2013;(9):28-30
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe effects of Naotaifang on MMP-9, NF-κB and TIMP-1 after focal cerebral ischemia in rats. Methods The rats were randomly divided into sham operation group, model group, and Naotaifang low- (3 g/kg), medium- (9 g/kg), high- dose (27 g/kg) group. After 3 days of corresponding therapy by intragastric administration once a day, the regional cerebral ischemia model was made by middle cerebral artery occlusion (MCAO) with suture method. Following 3 days, the rats were treated with previous method. On the third day, hippocampal C2 region of ischemic tissue was detected by HE dyeing. And the contents of MMP-9, NF-κB and TIMP1 proteins in hippocampal C2 region were measured by immunohistochemical method. Results The number of normal brain cells in high dose group of Naotaifang was more than that of the model group, and only a few cells appeared nucleus pycnosis. The MMP-9 expression of all dose groups of Naotaifang were significantly decreased than model group (P<0.05). The NF-κB expression of high and medium dose groups of Naotaifang were significantly decreased (P<0.05). The TIMP1 expression of all dose groups of Naotaifang were significantly increased compared with sham operation group (P<0.05). Conclusion The mechanism of Naotaifang protecting blood brain barrier against injury of cerebral ischemia may be involved in ameliorating MMP, NF-κB and increasing TIMP1 expression.
