Effect of Ruanmailing Oral Liquid on Angiogenesis in Atherosclerotic Plaque of Apoliporotein E Gene knock-out Mice
10.3969/j.issn.1005-5304.2013.09.013
- VernacularTitle:软脉灵口服液对ApoE基因敲除小鼠动脉粥样硬化斑块内血管新生的影响
- Author:
Tianmin WU
;
Jinshui CHEN
;
Xiyang ZENG
- Publication Type:Journal Article
- Keywords:
atherosclerotic plaque;
Ruanmailing Oral Liquid;
Apolipoprotein E gene knock-out mice;
angiogenesis
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2013;(9):37-39
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of Ruanmailing Oral Liquid on angiogenesis in atherosclerosis plaque of Apolipoprotein E (ApoE) gene knock-out mice, and explore the mechanisms of plaque stabilizing. Methods Totally 30 mice 6-8 weeks old ApoE knockout mice were fed a high fat diet for 12 weeks until the formation of a mature atherosclerotic plaque. They were randomly divided into three groups-model group, Ruanmailing group, simvastatin group, with another 6 normal C57BL/6J mice as the control group, and were administered for 12 weeks. Blood was extracted from orbital venous to measure the lipid (TC, TG, LDL-C, HDL-C) variation. HE-stain was used to observe aortic pathomorphological changes, meanwhile, immunohistochemical method was adopted to determine the microvessel density of plagues which is marked by CD105, as well as the expression of CD105 in aorta. Results Compared with the model group, TC, LDL-C, TG of Ruanmailing group and simvastatin group decreased significantly, while HDL-C increased significantly (P<0.01), degree of pathological damage was reduced, microvessel density and the expression of CD105 was significantly lower (P<0.01). There was no significant difference between Ruanmailing group and simvastatin group in above-mentioned indicators (P>0.05). Conclusion Ruanmailing Oral Liquid may reduce the expression of CD105 and inhibit the angiogenesis within the plaque, which is the possible mechanism of stabilizing the atherosclerotic plaque.
