Content Change of Aconitine in Radix Aconiti Kusnezoffii with Different Boiling Time
10.3969/j.issn.1005-5304.2013.09.016
- VernacularTitle:不同煎煮时间内生草乌中乌头碱含量变化研究
- Author:
Xuexiang LIU
;
Weiwei YAN
- Publication Type:Journal Article
- Keywords:
Radix Aconiti Kusnezoffii;
aconitine;
decocting time;
HPLC
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2013;(9):47-48
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the content change of aconitine in Radix Aconiti Kusnezoffii in different boiling time. Methods The chromatographic procedure was carried out with methanol-water-chloroform-diethylamide (70∶30∶3∶0.2) as mobile phase at a flow rate of 1.0 mL/min, the column temperature at 30 ℃, and UV detector at 240 nm. Results There was a good linear relationship of aconitine within 0.08-0.8 μg, r=0.999 8 (n=6), and the average recovery rate was 97.16%. The content of aconitine increased gradually in 0-2 h, then gradually decreased. And the reduction was evident particularly during 2-4 hours. Conclusion HPLC method is rapid, simple, accurate, reproducible and reliable for determining aconitine. The content of aconitine can be decreased effectively by boiling 4 hours.
