HIV-1 Nef promotes KSHV vIL-6-induced angiogenesis by regulating PTEN/PI3K signaling pathway

VernacularTitle:HIV-1 Nef蛋白通过调控 PTEN／PI3 K信号通路促进KSHV vIL-6诱导血管生成
Author: Shuihong YAO ; Xiaofei ZHU ; Chun LU ; Gaoxia GE
Publication Type:Journal Article
Keywords: KSHV; Negative factor (Nef); Viral interleukin-6 (vIL-6); PTEN/PI3K signaling pathway; Angiogenesis
From: Chinese Journal of Microbiology and Immunology 2013;(8):627-633
CountryChina
Language:Chinese
Abstract: Objective To investigate whether HIV-1 Nef could promote the angiogenesis and tu-morigenesis induced by KSHV vIL-6 through regulating PTEN/PI3K signaling pathway .Methods Lipo-some transfection was used to transfect cDNA of pPTEN , dominant-negative ( DN) construct of PI3K and control vector into endothelial cells , which stably express KSHV vIL-6 and HIV-1 Nef.Microtubule forma-tion assay and chicken chorioallantoic membrane ( CAM) assay were used to evaluate microtubule formation and angiogenesis , respectively .Expressions of PTEN and PI 3 K were measured by Western blot .Results Both overexpression of PTEN and inhibited expression of PI 3K suppressed the vIL-6-induced microtubule for-mation and angiogenesis in CAM mediated by Nef .Conclusion HIV-1 Nef enhances vIL-6-induced angio-genesis and tumorigenesis through regulating PTEN/PI3K signaling pathway .