Isolation,culture and identification of neonatal mouse cardiomyocytes
10.3969/j.issn.1671-8348.2013.22.026
- VernacularTitle:原代乳小鼠心肌细胞的分离培养及鉴定
- Author:
Kuoju CHENG
;
He HUANG
;
Zhiyong DU
;
Hong LI
;
Tiande YANG
- Publication Type:Journal Article
- Keywords:
neonatal mouse;
cardiomyocytes;
separation;
purification;
culture;
identification
- From:
Chongqing Medicine
2013;(22):2638-2640
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method to isolate,culture and identify cardiomyocytes from neonatal mouse,estimate the cardiomyocytes survival rate,and identify the purity of cardiomyocytes.Methods Neonatal C5 7 mouse heart(within 3 days)was di-gested using trypsin and collagenase.Cardiomyocytes survival rate was estimated by trypan blue staining,and cardiomyocytes purity was identified byα-actin immunofluorescence staining.Results Mouse cardiomyocytes could be successfully isolated and cultured using neonatal mouse within 3 days.Trypan blue staining showed cardiomyocytes survival rate was>95%,and cardiomyocytes pu-rity was more than 95% demonstrated byα-actin immunofluorescence staining.Conclusion Under the strict experimental condi-tions,mouse cardiomyocytes can be successfully isolated and cultured with high survival rate and high purity.
