Determination of berberine hydrochloride in modified Ermiao pill by HPLC
10.3760/cma.j.issn.1673-4246.2013.09.012
- VernacularTitle:RP-HPLC测定加味二妙丸中盐酸小檗碱的含量
- Author:
Mingdi ZHANG
- Publication Type:Journal Article
- Keywords:
modified Ermiao pill;
Berberine hydrochloride;
RP-HPLC
- From:
International Journal of Traditional Chinese Medicine
2013;35(9):806-808
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a HPLC method for the determination of berberine hydrochloride in Jiawei Ermiao Wan.Methods The RP-HPLC system consisted of the Luna-C18 (4.6 mm × 250 mm,5 μm) column,the mobile phase consisted of acetonitrile-0.1％H3PO4(45:55),the flow rate was 1.0mL · min-1,the column temperature was 30℃,The UV detector was set at 245nm.Results The linear response range was 0.071～1.42 mg· ml-1 (r=0.9998).The average recovery of mangiferin was 99.2％,and RSD＜2％.Conclusion The method was simple,rapid,accurate and repeatable.It could be applied in the determination of berberine hydrochloride in Jiawei Ermiao Wan.
