GC-MS determination of metabolites in rat kidneys
10.3969/j.issn.1672-7347.2013.07.002
- VernacularTitle:大鼠肾组织中小分子代谢物的GC-MS分析
- Author:
Shao LIU
;
Fangjie WANG
;
Wenjuan MEI
;
Lijian TAO
- Publication Type:Journal Article
- Keywords:
kidney tissue;
metabolite;
GC-MS;
chemometrics
- From:
Journal of Central South University(Medical Sciences)
2013;38(7):661-669
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a method to determine the metabolites in rat kidney tissues by gas chromatography-mass spectrometry (GC-MS) combined with chemometric techniques. Methods:Metabolites were separated and identiifed on HP-5MS column (30 m × 0.25 μm × 0.25 mm). The initial column temperature was 100℃lasting 3 min, and then programmed at 8℃/min to 300℃, maintaining at this temperature for 6 min. hTe internal standard was heptadecanoic acid. hTe grinded kidney tissue was exacted by methanol. hTe supernatant was dried by nitrogen. Atfer the oximation and derivation, the supernatant was analyzed by GC-MS. hTe overlapped peaks were resolved into pure chromatogram and mass spectra with chemometric techniques. Qualitative analysis was performed by comparing the obtained pure mass spectra with those in NIST mass spectra database and certiifcated by the standards and the references. hTe internal method was used for semi-quantitation. Results:A total of 53 compounds were identiifed. hTe main constitutions in the kidney tissue were amino acids, saccharides, fatty acids and urea. Conclusion:hTe combination of methods is rapid and accurate for the analysis of metabolites in the kidney tissue, which provides more information for further study of metabonomics in kidney tissues.
