Effects of Shuxuetong Injection on expressions of cell apoptosis and TLR 4 around ischemic area after focal cerebral infarction in rats
10.3969/j.issn.1671-8348.2013.25.022
- VernacularTitle:疏血通注射液对局灶性脑梗死大鼠缺血周围区细胞凋亡和TLR4表达的影响
- Author:
Yihua ZHONG
;
Guangqin LI
;
Xianjun TANG
- Publication Type:Journal Article
- Keywords:
cerebral infarction;
inflammatory reaction;
Shuxuetong
- From:
Chongqing Medicine
2013;(25):3011-3014
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of Shuxuetong Injection (SXT ) on expressions of cell apoptosis and TLR4 a-round ischemic area after focal cerebral infarction in rats and to discuss its neuroprotective mechanism on ischemia-induced brain in-jury .Methods The SD rats were subjected to establish the model of middle cerebral artery occlusion (MCAO)by nylon monofila-ment suture ,then were randomly divided into the sham-operated group ,the model group and the SXT treatment group ;the cell ap-optosis and the expressions of TLR4 mRNA and protein around ischemic area at 12 ,24 ,48 ,72 h after cerebral ischemia were detec-ted respectively by TUNEL test mediated with DNA ,RT-PCR and immunohistochemistry .Results In the model group ,the number of TUNEL positive cells ,the expressions of TLR4 mRNA and protein were gradually increased at 12 h ,reached the peak at 24 h , then decreased and were still higher than those in the sham-operated group(P<0 .01);in the SXT treatment group ,these expres-sions after 24 h were lower than those in the model group (P<0 .05)and declined as the treatment time increase(P<0 .05) .Conclu-sion In subacute stage of cerebral ischemia injury ,apoptosis is related with the expression of TLR4 ,SXT may inhibit apoptosis , down-regulate the expression of TLR4 around ischemic area ,this may be one of the mechanisms of neuroprotection .
